Journal: Scientific Reports

Article Title: USP8 modulates ubiquitination of LRIG1 for Met degradation

doi: 10.1038/srep04980

Figure Lengend Snippet: (a) LRIG1 protein levels in patient-derived lung tumor xenograft samples were measured by ELISA. Each symbol represents one tumor sample. (b) USP8 protein levels were measured by immunoblot in patient-derived lung tumor xenograft samples. (c) Met protein levels in patient-derived lung tumor xenograft samples were measured by ELISA, and expressed as a percentage (%) relative to the amount of Met in vehicle-treated tumor. Asterisks (*) represent P-values according to Student's t test, *P < 0.02, **P < 0.0001. (d) Patient-derived lung tumor growth over time. Each group consists of 10 mice. Tumor volumes measured on indicated days are plotted (mean and s.e.m.) for treatment groups (SAIT301) and vehicle (negative control: PBS) group. Asterisks (*) represent P-values versus vehicle group according to repeated measures ANOVA (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001).

Article Snippet: For the sandwich ELISA, total Met levels in homogenized tumor lysates were determined using human total HGF R/c-Met ELISA kit (R&D systems).

Techniques: Derivative Assay, Enzyme-linked Immunosorbent Assay, Western Blot, Negative Control